Journal: Nucleic acids research

Article Title: Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.

doi: 10.1093/nar/gkz1151

Figure Lengend Snippet: Figure 1. Local RNA in situ hybridization. (A) Schematic overview of a microfluidic implementation of chromogenic RNA in situ hybridization. The initial preparation of the tissue section including paraffin removal (1), heat (2) and protease (3) pretreatment, as well as fixation was performed globally. The primary probe was then hybridized locally to the RNA of interest (4). In this case the detection of HER2 is shown with internal negative (dapB) and positive (ActB) controls. Finally, the amplification of the signal using preamplifier and amplifier strands (5), the binding of enzyme (6), and the enzymatic reactions (7) were performed globally on the whole slide. (B) Fluorescence images of RNA-ISH experiments detecting the gene expression of HER2 in cell pellet sections of the breast cancer cell lines MCF7 (left), SKBR3 (center) and BT474 (right). Probes directed against bacterial dapB and ActB were used as negative and positive controls, respectively. Scale bar: 100 m.

Article Snippet: FFPE cell blocks and tissues FFPE cell blocks of the cell lines MCF7, BT474 and SKBR3 (AMS Biotechnology, Abingdon, UK) with HER2 expression levels of 1+, 2+ and 3+, respectively, were used as references.

Techniques: RNA In Situ Hybridization, Amplification, Binding Assay, Fluorescence, Gene Expression

Journal: Nucleic acids research

Article Title: Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.

doi: 10.1093/nar/gkz1151

Figure Lengend Snippet: Figure 2. Detection of HER2 expression status by RNA in situ hybridization using internal positive and negative controls. (A) Fluorescence images of the signals detected for HER2, dapB, and ActB in a mammary carcinoma section. Scale bar: 100 m. (B) Quantitative analysis of the fluorescence intensity of FISH signals detected for HER2, dapB, and ActB integrated per sectioned cell for FFPE sections of the breast cancer cell lines MCF7, SKBR3, and BT474 (see Figure 1) as well as the mammary carcinoma from (A). 100 ms excitation.

Article Snippet: FFPE cell blocks and tissues FFPE cell blocks of the cell lines MCF7, BT474 and SKBR3 (AMS Biotechnology, Abingdon, UK) with HER2 expression levels of 1+, 2+ and 3+, respectively, were used as references.

Techniques: Expressing, RNA In Situ Hybridization, Fluorescence

Journal: Nucleic acids research

Article Title: Spatially multiplexed RNA in situ hybridization to reveal tumor heterogeneity.

doi: 10.1093/nar/gkz1151

Figure Lengend Snippet: Figure 3. Single color multiplexed RNA-ISH for the detection of breast cancer biomarkers. (A) The primary probes for ER, PgR, and HER2 were delivered to spatially distinct regions of a mammary carcinoma section using a microfluidic chip (schematic in upper left corner). Fluorescence images of the signal detected for the breast cancer biomarkers ER, PgR and HER2 in a mammary carcinoma section. Scale bar: 100 m. (B) Fluorescence intensity of FISH signals detected for ER, PgR and HER2 integrated per sectioned cell for FFPE sections of the breast cancer cell lines MCF7, SKBR3 and BT474 (see Supplementary Figure S7) as well as the mammary carcinoma from (A). 500 ms excitation.

Article Snippet: FFPE cell blocks and tissues FFPE cell blocks of the cell lines MCF7, BT474 and SKBR3 (AMS Biotechnology, Abingdon, UK) with HER2 expression levels of 1+, 2+ and 3+, respectively, were used as references.

Techniques: Fluorescence

Journal:

Article Title: High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides

doi: 10.1136/jcp.2003.013029

Figure Lengend Snippet: Microarray comparative genomic hybridisation (CGH) profile of the BT474 cell line. (A) Bacterial/phage artificial chromosome polymerase chain reaction representations were used as probe DNAs spotted on to the glass slide; (B) oligonucleotides were used as probe DNAs spotted on to the glass slide. Moving average applied to the log2 ratio of the oligo CGH profile. The vertical bars indicate the spacing between the chromosomes.

Article Snippet: Genomic DNA was isolated from normal kidney (female donor), blood (male donor), or liver (female donor) as a reference, and from the breast cancer cell line BT474, according to Snijders et al . 7 The DNA from the GM00143 cell line (Coriell Institute for Medical Research, Camden, New Jersey, USA) was provided by the Albertson laboratory (University of California, San Francisco, USA).

Techniques: Microarray, Hybridization, Polymerase Chain Reaction

Journal:

Article Title: High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides

doi: 10.1136/jcp.2003.013029

Figure Lengend Snippet: Microarray comparative genomic hybridisation (CGH) profile of the long arm of chromosome 17 of the BT474 cell line. Grey triangles, bacterial/phage artificial chromosome polymerase chain reaction representations were used as probe DNAs spotted on to the glass slide; black squares, oligonucleotides were used as probe DNAs spotted on to the glass slide (log2 ratio without moving average). The horizontal bars indicate the two separate amplicons observed.

Article Snippet: Genomic DNA was isolated from normal kidney (female donor), blood (male donor), or liver (female donor) as a reference, and from the breast cancer cell line BT474, according to Snijders et al . 7 The DNA from the GM00143 cell line (Coriell Institute for Medical Research, Camden, New Jersey, USA) was provided by the Albertson laboratory (University of California, San Francisco, USA).

Techniques: Microarray, Hybridization, Polymerase Chain Reaction

Journal: BioMed Research International

Article Title: Preclinical and Clinical Effects of Mistletoe against Breast Cancer

doi: 10.1155/2014/785479

Figure Lengend Snippet: Summary of in vitro and in vivo studies of mistletoe extracts on breast cancer cells and animal models.

Article Snippet: In 2006, Beuth et al. [ ] studied the human ductal breast carcinoma cell line BT474 and proved the direct antitumor efficacy of Helixor M and Helixor A using a calorimetric in vitro assay.

Techniques: In Vitro, In Vivo, Activity Assay, Purification, Inhibition, Transplantation Assay

Journal: American Journal of Cancer Research

Article Title: KRAS signaling enriched triple negative breast cancer is associated with favorable tumor immune microenvironment and better survival

doi:

Figure Lengend Snippet: KRAS signaling was functioning in the breast cancer cell lines. A. Up-regulation of KRAS expression in breast cancer cell lines when compared with MCF10A. Densitometric values were calculated for KRAS. B. Cell growth suppression after transfection of MB231 cells with MEK inhibitor or PI3K inhibitor at 72 H. Comb: PI3Ki 1 + MEKi 5 (μM). *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Cell culture MCF10A, MCF7, SKBR3, BT474, and MB231 cells were obtained from the JCRB (Japanese Collection of Research Bioresources) Cell Bank.

Techniques: Expressing, Transfection